Sanger Sequencing DNA fragments
I am a PhD student, doing work mainly on bioinformatics, thus my simple question that follows.
For my work I will need to perform Sanger Sequencing to confirm the presence of multiple different SNVs in a sample of individuals with a given disorder.
To confirm a single SNV by Sanger, I first have to amplify a DNA fragment, from the genomic samples of the individuals, which will be done by conventional PCR. For the sequencing itself, where we expose the obtained fragment, separately, to forward and reverse primers, is it correct to use the same pair of primers I used for the conventional PCR? Since the region complementary to the primers will still be in the fragment, I suppose this is ok.
Sorry for my really basic question.
Thanks in advance