Magnetic Bead-Based RNA Extraction
I am working on a Microfluidic Magnetic bead based Assay with an extraction method for RNA. The beads used are coated with a silica coating, and will be extracted using an elution buffer. MY background in my molecular biology only extends to orgo chem separation techniques such as Trizole digest with chloroform or phenol extraction, and precipitation with 100% IPA. I am curious to know if anyone has had any experience with magnetic bead based RNA extraction. I am having trouble determining the appropriate elution buffer pH, does anyone know an optimal pH for silica bead elution. I would think it is similar to a column elution pH. Thank you!
Any feedback I will appreciate!