RNAlater (R) and collagenase interaction
I am working with a difficult to homogenize tissue, and was wondering if digestion with collagenase would aid in cell separation and lysis. I store my samples in RNAlater or RNAlater ICE as needed. I am working with samples 10-30mg in size and have been frustrated with liquid nitrogen. If I freeze a sample immediately after collection, I have little RNA degradation, but when later isolating, getting an accurate weight is confounded with condensation and ice from liquid nitrogen temperatures.
Does anyone know how collagenase and RNAlater would interact if incubated before or after storing in RNAlater?