Which purification protocol is the best?
Given purification of the same enzyme using 4 diffrerent protocols, based on the concentration, volume and total activity of enzyme from each protocol, which is the best? What do i use as an indicator as to which protocol is the best? Specific enzyme activity or yield?
Protocol A: 100uL, 20mg/mL, Total activity: 100units/s
Protocol B: 100uL, 50mg/mL, Total activity: 10units/s
Protocol C: 500uL, 20mg/mL, Total activity: 100units/s
Protocol D: 100uL, 10mg/mL, Total activity: 1000units/s