anti-avidin antibodies in normal sera
I am curious if anyone has similar experience. I am observing a lot of anti-avidin (or more specificaly NeutrAvidin from Pierce) antibodies in normal human sera. I am trying to devise an assay that uses a biotinylated antigen but I can't seem to loose the background signal. I don't want to dilute my target sera too much so as to be able to observe low affinity specific Abs as well.
Thanks for the feedback.