stratalinker: Oligo Damage?
Hi, Does any one have any experience with the stratalinker? I am looking to immobilise a fluorophore labelled ss Oligo (~20-30bp).
It is standard in our lab to immobilize at 1 j/cm2, does anyone know of any data to suggest this could result in DNA damage for such short ss oligos? Of course, I want to be confident that when quantifying using my fluorophore, I am not under estimating due to the fact some oligos are damaged and therefore their fluorophore washed away.