Paralens fluorescent attachment to detect DAPI and Hoechst?
As a cheap alternative to a fluorescence microscope for my home lab, I am considering the Paralens Advance screw-on objective lens.
(Please see page 9 of the PDF for an illustration of working spectrum)
The Paralens produces excitation at 385-480nm and emission at 480nm (as far as I can tell, please see PDF above!). Question: Will this work with Hoechst 33258 and DAPI stain?
- Hoechst 33258: excitation: 352, emission: 461
- DAPI: excitation: 358, emission: 461