I recently submitted a paper and one of the reviewer wants to have the RIN number for my RNA. How can I calculate or measure RIN number? I looked on the web but it says I need a software and equipment to calculate. Can it be done without it, as we don't have the necessary software to do the same.
Also, the reviewer says what primers were used to make the cDNA. I didn't use primers (one step qPCR) but used a cDNA kit to generate my cDNA from the RNA. Can I say that I used a two-step qPCR protocol i.e. I generated my cDNA using a kit rather than primers?