Re-extracting proteins from pellet
I am extracting proteins from rat hippocampus and using it for Western Blot (WB). I'm using RIPA buffer to extract the proteins, however, I'm out of my protein sample now. I saved the pellet, from my 1st extraction. Can I use the pellet again to re-extract proteins using the same method as I did before, i.e. adding RIPA and Halt-protease inhibitor?