Counting number of cells
I am new to cell culture and require some help regarding understanding confluency, cell counting, and transfection.
1) I would like to know how can we determine the % of cell confluency before or after sub-culturing?
2) How do I count the number of cells?
3) How do I "Plate 5 x 104 cells per well in 0.5 ml of complete growth medium"?
4) If you have a protocol for cell transfection, could you please share it? How do I measure transfection efficiency? Do I always have to do an immuno to determine the efficiency?